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Saturday, 23 November 2013

The Tamil Nadu Dr. MGR Medical University Past 25 years Question Paper Key - Oral Histology

Development of teeth

Mention various stages of tooth development. Describe in detail the life cycle of ameloblast with diagrams: I. Introduction-significance of having a thorough knowledge about tooth development - formation of primary epithelial band (embryological origin/initiation of tooth - development, primary epithelial band, dental lamina, vestibular lamina) - list of the stage of tooth development - 2. Description of the developmental stages in detail - Bud stage (with diagram) - Cap stage (with diagram) - Early bell stage (with diagram) - Advanced bell stage (Breakdown of dental lamina, hard tissue formation, crown - pattern determination/morphodifferentiation and root formation with diagram) - 3. Life-cycle of ameloblast/Amelogenesis - Definition/what is amelogenesis - Light microscopic electron microscopic description of ameloblast during amelogeneis - electron microscopic description of ameloblast during amelogeneis/ Stages in amelogenesis (morphogenic, organizing , formative, maturative, protective and  desmolytic stages - with diagrams for each  stage) - note on the enamel proteins secreted - Clinical significance

Describe various stages of tooth development - 1. Introduction-significance of having a thorough knowledge about tooth development - formation of primary epithelial band (embryological origin/initiation of tooth-development, primary epithelial band, dental lamina, vestibular lamina) - list of the stages of tooth development- 2. Description of the developmental stages in detail - Bud stage (with diagram) - Cap stage (with diagram) - Early bell stage (with diagram) - Advanced bell stage (Breakdown of dental lamina, hard tissue formation, crown pattern determination/morphodifferentiation and root formation with diagram) - 3. Histophysiology of tooth development.- 4. Nerve and vascular supply during early development.- 5. Summary/Conclusion (note on: formation of permanent dentition-eruption and supporting      structures, timeline of tooth development with diagram of ontogeny of tooth)     and clinical considerations (anodontia, supernumerary teeth, enamel hypoplasia, dilacerations,     accessory/supernumerary roots.

Describe the bell stage of tooth development with the help of diagrams and add a note on root development - Tooth development - introduction - list the stages of tooth development - overview of bell stage of tooth development - 2. Description of the Bell Stage - Early bell stage (describe inner enamel epithelium, stratum intermedium, stellate reticulum, outer enamel epithelium, dental lamina, dental papilla, dental sac-with diagram) - Advanced bell stage (breakdown of dental lamina, mineralization and crown- pattern determination/morphodifferentiation, hard tissue formation  with diagram) - 3. Root formation - Root formation-an overview/introduction  - Hertwig’s Epithelial Root Sheath(formation, function/role in root formation, discovered by, general features and fate of HERS with diagram) - Root dentinogenesis/Root dentin formation – Cementogenesis - Formation of single rooted tooth (with diagram) - Formation of multirooted tooth (with diagram) - Root development and eruption - 4. Clinical significance (enamel hypoplasia, enamel pearl formation, accessory root development, dilaceration)

Enumerate the various stages of tooth development. Describe in detail advanced bell stage - 1. Introduction-significance of having a thorough knowledge about tooth development - formation of primary epithelial band (embryological origin/initiation of tooth - development, primary epithelial band, dental lamina, vestibular lamina) - list of the stages of tooth development - 2. Description of the developmental stages in detail - Bud stage (with diagram) - Cap stage (with diagram) - Early bell stage (with diagram) - Advanced bell stage – overview - 3. Advanced bell stage - Breakdown of dental lamina - Crown pattern determination / morphodifferentiation - Hard tissue formation (describe reciprocal induction, formation of enamel and dentin from ameloblast and odontoblast respectively with diagram) - Root formation (formation of Hertiwig’s epithelial root sheath and its role in root formation, root dentinogenesis and cementogenesis, formation of single rooted tooth, formation  of multirooted tooth and note on the fate of HERS with diagrams) - Clinical consideration (enamel pearl formation, accessory root development)

Write an essay on tooth development. Add a note on the histodifferentiation and morphodifferentiation of odontogenesis: I. Introduction-significance of having a thorough knowledge about tooth development - formation of primary epithelial band (embryological origin/initiation of tooth -  development, primary epithelial band, dental lamina, vestibular lamina) - list of the stages of tooth development - 2. Description of the developmental stages in detail - Bud stage (with diagram) - Cap stage (with diagram) - Early bell stage (with diagram) - Advanced bell stage (Breakdown of dental lamina, hard tissue formation, crown - pattern determination/morphodifferentiation and root formation with diagram) - 3. Histophysiology of tooth development. - Development of tooth based on histophysiological process ( overview of intiation, proliferation, histodifferentiation, morphodifferentiation and apposition) - Histodifferentiation (What is histodifferentiation/definition, differentiation of ameloblast, differentiation of odontoblast, reciprocal induction with diagram) - Morphodifferentiation (What is morphodifferentiation, establishment of dentinoenamel junction and dentinocemental junction and their role in determining crown pattern, incremental pattern of enamel and dentin deposition along DEJ and Dentinocemental junction with diagram) - 4. Summary/Conclusion (note on: nerve and vascular supply during early development,     formation of permanent dentition-eruption and supporting structures, timeline of tooth     development with diagram of ontogeny of tooth) and clinical considerations (anodontia, supernumerary teeth, enamel hypoplasia, dilacerations, accessory/supernumerary roots)

Describe the development of roots of maxillary permanent molars:- 1. Introduction - no. and name (Max: MB, DB, P) of roots of maxillary permanent molars - chronology of maxillary permanent molars (first evidence of calcification, eruption and root completion) - 2. Root development in maxillary permanent molars - I. formation of HERS – role in root formation (with diagram) - 2. root dentin development - 3. epithelial diaphragm – role in root formation in the case of three rooted maxillary permanent molars (with diagram) - 3. Clinical Considerations (supernumerary roots, dilacerations)

Functions of stellate reticulum: What is stellate reticulum? - location of these cells in the enamel organ - shape of the cells (role of glycosaminoglycans in determining the shape) - 2. Diagram - 3. Functions of stellate reticulum/role of stellate reticulum in tooth development (cushioning effect, shock absorption, protection, makes enamel organ resistant and elastic)- role of enzyme - 4. Fate of stellate reticulum prior to tooth eruption

Fate of dental lamina: I. What is dental lamina?-  primary epithelial band and formation of dental lamina diagram - 2. Fate of dental lamina - disintegration of dental lamina with diagram - eruption of teeth into the oral cavity (natural break in the continuity of the oral epithelium) 3. Clinical consideration - enamel pearl  – odontome/eruption cyst/ supernumerary teeth -  gingivitis and periodontitis (integrity of junctional epithelium compromised)

Development of pulp: 1. Role of dental papilla in the formation of dental pulp -  
description of cells in dental papilla - differentiation of mesenchymal cells into fibroblast and other pulp components 2. Embryological origin/ initiation of development of dental pulp during embryogenesis 3. Formation of pulp organ after differentiation of odontoblast and ameloblast - formation of cells - establishment of capillaries and nerve fibres - 4. Diagram - 5. Clinical consideration-growth factors produced for innervations of pulp during development

Hertwig’s epithelial root sheath: 1. Formation; discovered by/named after - 2. General features/description with diagram - 3. Function/role in root formation-in single rooted tooth, in multirooted tooth with diagram - 4. Fate of HERS with diagram-cell rests of Malassez - 5. Clinical consideration (supernumerary roots, dilaceration)

Cell rests of Malassez: 1. Formation (from HERS); discovered by/named after 2. Arrangement and distribution - 3. Description of the cells - Light microscopic and electron microscopic appearance - 4. Diagram - 5. Clinical Consideration (tumor, cyst, cementicles)

Histodifferentiation and morphodifferentiation of ameloblast: 1. Introduction/overview of histodifferentiation and morphodifferentiation in tooth development - 2. Histodifferentiation - what is histodifferentiation/definition, - differentiation of ameloblast, differentiation of odontoblast, reciprocal induction - 3. Morphodifferentiation - What is morphodifferentiation -  establishment of dentinoenamel junction and dentinocemental junction- crown pattern determination and  incremental pattern of enamel and dentin deposition- 4. Diagrams (for histodifferentiation and morphodifferentiation) - 5. Clinical Considerations (enamel hypoplasia, atypical dentin, supernumerary cusp, peg shaped teeth, dilaceration, supernumerary roots).

Bell stage: 1. Bell stage in tooth development – introduction/overview 2. Early bell stage (describe inner enamel epithelium, stratum intermedium,  stellate reticulum,     outer enamel epithelium, dental lamina, dental papilla, dental sac-with diagram) 3. Advanced bell stage (breakdown of dental lamina, mineralization and crown pattern  determination/morphodifferentiation, hard tissue formation  with diagram)- 4. Clinical Considerations (enamel hypoplasia, atypical dentin, supernumerary cusp, peg shaped teeth, dilacerations, supernumerary roots).

Late Bell stage: 1. Bell stage in tooth development – introduction; breakdown of dental lamina 2. Hard tissue formation (describe reciprocal induction, formation of enamel and dentin from     ameloblast and odontoblast respectively/histodifferentiation with diagram)- 3. Crown pattern determination / morphodifferentiation with diagram - 4. Root formation (formation of Hertiwig’s epithelial root sheath and its role in  root formation,  root dentinogenesis and cementogeneis, formation of single rooted tooth, formation  of multirooted tooth and note on the fate of HERS with diagrams) - 5. Clinical consideration (enamel pearl formation, accessory root development)

Epithelial mesenchymal interaction in tooth development: 1. Introduction-what is epithelial mesenchymal interaction? - 2. Epithelial mesenchymal interaction in the differentiation of ameloblast and odontoblast with diagram - 3. Epithelial mesenchymal interaction in the formation of root dentin and cementum with  diagram. - 4. Epithelial mesenchymal interaction in the formation of supporting tissues of the tooth i.e. periodontal ligament and alveolar bone - 5. Clinical considerations (enamel hypoplasia, osteodentin) and molecular insights (signal involved in epithelial mesenchymal interactions)




Enamel

Describe life cycle of ameloblasts: Stages of ameloblasts – 6 stages – differentiation from dental lamina - oral epithelium – formation of bud – differentiation of inner and outer cells – changes in organelles, form, shape, size and function. Reciprocal induction – genetic control – interplay of growth factors and differentiation factors - With diagrams

Microscopic feature of enamel: Composition of enamel – organic, water and inorganic. Feature of enamel of rods – Light microscopy, Electron microscopy. Direction of enamel, number, ends of rods. Structures observed in enamel – lamellae, tufts, spindles, DEJ, Hunter-Schreuger Bands, Incremental lines, Gnarled enamel, dead tract - Diagrams

Age changes in enamel: Physical changes- in constitution, water content, amount of surface mineralization, regressive changes in enamel, cracks. Chemical constitution – impact of surface re-mineralization, Caries incidence   

Enamel lamellae: What is it – where – why – how – what does it cause- Role of stress – effect of enamel lamella - Diagrams

Dentinoenamel junction: where is it – shape – number – convexity facing – why corrugated. Structures associated with DEJ – what makes DEJ unique – separation – membrane preformitiva – clinical correlation- Diagrams  

Enamel tufts: what is it – where – why – how – what does it cause- Role of stress – effect of enamel tufts – regular – change in organic composition – fault zone - Diagrams  

Gnarled enamel: what is it – where – why – how – what does it cause- Optical illusion - Role of stress – how it supports in dissipating the force - Diagrams

Histological structure of enamel: Composition of enamel – organic, water and inorganic. Feature of enamel of rods – Light microscopy, Electron microscopy. Direction of enamel, number, ends of rods. Structures observed in enamel – lamellae, tufts, spindles, DEJ, Hunter-Schreuger Bands, Incremental lines, Gnarled enamel, dead tract, Neo-natal ring – Nasmyth’s membrane - Diagrams

Ameloblasts: Cell of origin – how inner enamel epithelium turns to ameloblasts – reversal of polarity – inductive effect - change in position of organelles – life cycle of ameloblast – secretion – stages – processes – nutrition source – desmolysis - Diagrams

Enamel spindle: What is it – where – why – how – what does it cause- types - extension – effects of extension – clinical significance - Diagrams

Enumerate hypocalcified areas of enamel and describe enamel lamellae, tufts. Give their clinical significance: How there is defective or decreased calcification – lamellar, tufts, spindles, DEJ. Enamel lamellae: What is it – where – why – how – what does it cause- Role of stress – effect of enamel lamella - Dentinoenamel junction: where is it – shape – number – convexity facing – why corrugated. Structures associated with DEJ – what makes DEJ unique – separation – membrane preformitiva – clinical correlation  - Enamel tufts: what is it – where – why – how – what does it cause- Role of stress – effect of enamel tufts – regular – change in organic composition – fault zone - Enamel spindle: what is it – where – why – how – what does it cause- types - extension – effects of extension – clinical significance - Diagrams

Lines of enamel: How lines are formed – incremental lines – daily rhythm – neonatal ring; Bands – Hunter Scherger Bands: Uses – clinical implication – age estimation – tetracycline etc., - Diagrams

Enamel rods: Structure, direction, number, size, shape, degree of calcification. Appearance under light microscope and electron microscope, crystals orientation, direction of crystals. Clinical significance of enamel rod direction – difference between primary and secondary dentition; acid etching - Diagrams

Describe microscopic appearance of enamel: Composition of enamel – organic, water and inorganic. Feature of enamel of rods – Light microscopy, Electron microscopy. Direction of enamel, number, ends of rods. Structures observed in enamel – lamellae, tufts, spindles, DEJ, Hunter-Schreuger Bands, Incremental lines, Gnarled enamel, dead tract, Neo-natal ring – Nasmyth’s membrane - Structure, direction, number, size, shape, degree of calcification. Appearance under light microscope and electron microscope, crystals orientation, direction of crystals. Clinical significance of enamel rod direction – difference between primary and secondary dentition; acid etching - Diagrams

Describe structure of mature enamel: Composition of enamel – organic, water and inorganic. Feature of enamel of rods – Light microscopy, Electron microscopy. Direction of enamel, number, ends of rods. Structures observed in enamel – lamellae, tufts, spindles, DEJ, Hunter-Schreuger Bands, Incremental lines, Gnarled enamel, dead tract, Neo-natal ring – Nasmyth’s membrane - Structure, direction, number, size, shape, degree of calcification. Appearance under light microscope and electron microscope, crystals orientation, direction of crystals. Clinical significance of enamel rod direction – difference between primary and secondary dentition; acid etching - Diagrams

Maturation of enamel: Stages of differentiation – Maturation phase – final changes in ameloblasts – changes in enamel matrix – calcification process – Tomes granular process – Reduced enamel epithelium – desmolytic changes – clinical significance.

Hunter-Scherger bands: What is it – where – why – how – what does it cause- why the color  and optics - clinical significance - Diagrams

Incremental lines of Retzius: Structure, direction, number, size, shape, degree of calcification. Appearance under light microscope and electron microscope, crystals orientation, direction of crystals. Clinical significance of – difference between primary and secondary dentition; Neonatal ring- Diagrams

Describe amelogenesis with suitable diagrams: Stages of ameloblasts – 6 stages – differentiation from dental lamina - oral epithelium – formation of bud – differentiation of inner and outer cells – changes in organelles, form, shape, size and function. Reciprocal induction – genetic control – interplay of growth factors and differentiation factors - With diagrams – Morphogenetic, histodifferentiation etc.,

Discuss in detail the life cycle of ameloblast: Stages of ameloblasts – 6 stages – differentiation from dental lamina - oral epithelium – formation of bud – differentiation of inner and outer cells – changes in organelles, form, shape, size and function. Reciprocal induction – genetic control – interplay of growth factors and differentiation factors - With diagrams – Morphogenetic, histodifferentiation etc.,

Enumerate with suitable diagrams the microscopic structure of enamel: Composition of enamel – organic, water and inorganic. Feature of enamel of rods – Light microscopy, Electron microscopy. Direction of enamel, number, ends of rods. Structures observed in enamel – lamellae, tufts, spindles, DEJ, Hunter-Schreuger Bands, Incremental lines, Gnarled enamel, dead tract, Neo-natal ring – Nasmyth’s membrane - Structure, direction, number, size, shape, degree of calcification. Appearance under light microscope and electron microscope, crystals orientation, direction of crystals. Clinical significance of enamel rod direction – difference between primary and secondary dentition; acid etching - Diagrams

Life cycle of ameloblast: Stages of ameloblasts – 6 stages – differentiation from dental lamina - oral epithelium – formation of bud – differentiation of inner and outer cells – changes in organelles, form, shape, size and function. Reciprocal induction – genetic control – interplay of growth factors and differentiation factors - With diagrams – Morphogenetic, histodifferentiation etc.,

Hypocalcified structures of enamel     : How there is defective or decreased calcification – lamellar, tufts, spindles, DEJ. Enamel lamellae: What is it – where – why – how – what does it cause- Role of stress – effect of enamel lamella - Dentinoenamel junction: where is it – shape – number – convexity facing – why corrugated. Structures associated with DEJ – what makes DEJ unique – separation – membrane preformitiva – clinical correlation  - Enamel tufts: what is it – where – why – how – what does it cause- Role of stress – effect of enamel tufts – regular – change in organic composition – fault zone - Enamel spindle: what is it – where – why – how – what does it cause- types - extension – effects of extension – clinical significance - Diagrams

Morphodifferentiation of enamel organ: Stages of ameloblasts – 6 stages – differentiation from dental lamina - oral epithelium – formation of bud – differentiation of inner and outer cells – changes in organelles, form, shape, size and function. Reciprocal induction – genetic control – interplay of growth factors and differentiation factors - With diagrams – Morphogenetic, histodifferentiation etc.,- abnormalities associated with morphodifferentiation.




Dentin

Osteodentin: define – types of tertiary dentin – rapidity of production – external/internal stimulus – trapping of new/old odontoblast – appearance – clinical significance; Diagram

Age changes in dentin: Macroscopic changes - color, size, thickness, regressive alteration, sensitivity; Microscopic changes – dead tracts, eburnation, sclerotic dentin – Changes in constitution – Inorganic, organic

Describe histology and functions of dentin: dentinal tubules – shape, microscopic and submicroscopic structures – intertubular, intratubular, Hypocalcified areas of dentin, course of dentinal tubules, types of dentin – primary, secondary, tertiary, mantle, circumpulpal, predentin; Functions – reciprocal induction, secretive, nutritive, sensory, protective, semipermeable, reparative; Diagram

Sensitivity of dentin: defects in enamel – how chemical alterative in oral environment reaches dentin – odontoblastic fibers in DEJ – Mechanism of sensing – three theories – direct innervations; transduction;  hydrodynamic theory – clinical significance. Diagram

Sensitivity of dentin theories: Mechanism of sensing – three theories – direct innervations; transduction;  hydrodynamic theory – clinical significance. Diagram

Interglobular dentin: Mineralization of dentin – spheres of calcification – fusion of globules – appearance – color – position of interglobular dentin

Dead tracts: Appearance – reason – when, where, how, why – clinical significance

Types of dentin: time of formation – primary/secondary/ tertiary and place – predentin/circumpulpal/mantle dentin: microscopic – inter/intra/peri tubular dentin; Sclerotic dentin/eburnated dentin - diagrams

Reparative dentin: type of stimulus – reactive/reparative dentin – odontoblast differentiation – difference between normal and reparative dentin ultra-structure and composition - significance

Innervations of dentin: odontoblastic process – plexus of Raskhow – enamel spindles – theories of dentinal hypersensitivity – DEJ – pain pathway – types of nerve endings in dentin- pulp complex - clinical implication

Inter and peritubular dentin: Ultra-structure of dentinal tubule – lamina limitans – inter and intra tubular dentin – peritubular dentin – difference in composition – rate of mineralization – function – origin – clinical implication including acid etching

Mantle dentin: place of formation – difference between predentin – Mantle dentin in composition – collagen fiber variation – strength – clinical implication
Enumerate various types of dentin. Describe primary dentin: time of formation – primary/secondary/ tertiary and place – predentin/circumpulpal/mantle dentin: microscopic – inter/intra/peri tubular dentin; Sclerotic dentin/eburnated dentin – diagrams. Roots formation – how root formation cause shift in course of dentinal tubule – line separating

Sclerotic dentin: age change in dentin – death of odontoblastic process – retraction – space – mineralization – Refractive index – similarity – clinical implication

Dentinal tubules: constituents – direction – micro and macroscopic structure – no. of tubules per sq mm; types -  fluid – C.S. of dentin diagram; collagen and mineral variation – clinical implication

Primary and secondary curvatures of dentinal tubules: root formation – difference in direction of dentinal tubules – line separating the primary and secondary curvature – neonatal ring – curves at terminal ends

Odontoblasts: cells of papilla – reciprocal induction – differentiation of odontoblasts – factors stimulating – life long secretion – activity – functions of odontoblasts – reparative function

Secondary and tertiary dentin: time of formation – primary/secondary/tertiary dentin – difference in curves – inorganic constituents – rate of formation – difference in collagen, direction and orientation of fibers

Theories of dentin sensitivity: Mechanism of sensing – three theories – direct innervations; transduction;  hydrodynamic theory – clinical significance. Diagram

Hypocalcified structures of dentin: DEJ, Interglobular dentin,  Enamel Lamellae Type C, Cracks – mechanism of formation – where- why – when – how ?

Denticles: Definition - location - classification - etiology  -  true & false denticles - pulp stones  - free - attached - embedded  -  clinical considerations   -  diagram

Odontoblatic process: morphology – extension of cell body  - extent – function, size, direction, orientation with dentinal tubules,dental lymph/fluid, theories of hypersensitivity, plexus of Rashkow, extension in to enamel – clinical significance

Tome's granular layer: radicular dentin – cementum – dentin junction – bending and looping of tubule – reason optical appearance under LM - Diagrams

Explain dentinogenesis: Definition – location – differentiation of odontoblast from papilla cells – reciprocal induction – secretion of dentin matrix – calcification of matrix – Matrix vesicle theory – Interglobular dentin

Write in detail about the structure of dentin. Add a note on age changes of dentin. dentinal tubules – shape, microscopic and submicroscopic structures – intertubular, intratubular, Hypocalcified areas of dentin, course of dentinal tubules, types of dentin – primary, secondary, tertiary, mantle, circumpulpal, predentin; Functions – reciprocal induction, secretive, nutritive, sensory, protective, semipermeable, reparative; Diagram
Age changes in dentin: Macroscopic changes - color, size, thickness, regressive alteration, sensitivity; Microscopic changes – dead tracts, eburnation, sclerotic dentin – Changes in constitution – Inorganic, organic

Discuss the microscopic structure of dentin: dentinal tubules – shape, size, number, types, tubules direction, structure with diagram

Pain transmission in dentin: Plexus of Rashkow – Dentin – pulp complex, dentin fluid - Theories of dentin sensitivity: Mechanism of sensing – three theories – direct innervations; transduction;  hydrodynamic theory – clinical significance. Diagram





Cementum

Cellular cementum: Definition of cementum - physical characteristics - chemical composition  -  organic, inorganic water content   -  location - cementoblast  and cementocyte  -  morphology& function  - fibres in cellular cementum  -  functions & incremental lines. Diagram

Cementum: Definition of cementum - physical characteristics - chemical composition  -  organic, inorganic water content – classification - acellular &cellular  -  fibres in cellular  and acellular cementum  -  location - cementoblast  and cementocyte  -  morphology& function  - functions of cementum  -   incremental lines –CEJ  -   Diagram

Types of cementum: Definition of cementum - physical characteristics - chemical composition–classification based on presence of cementocytes & type of fibre present - acellular &cellular  -  fibres in cellular  and acellular cementum  -  location - cementoblast  and cementocyte  -  morphology& function – Hylaine layer – Intermediate cementum - incremental lines –CEJ—differences between AEFC &CIFC -  Diagrams

Cementogenesis: Definition - Hertwig’s epithelial root sheath  - origin of cementoblast—morphology and function - processes involved in cementogenesis - Development of cellular and acellular cementum - Mineralisation - matrix formation - attatchment of fibres—incremental line formation - cementocytes—Diagram

Hypercementosis: Definition – etiology (physiological  -  & pathological) - location - classification (localised/extensive ; single tooth/many) - excementosis - clinical considerations (extraction of teeth) - Diagram.

Excementosis: Definition - etiology(physiological  -  & pathological) - location - classification (localised/extensive ; single tooth/many) - clinical considerations (extraction of teeth)

Cementocytes: Origin—morphology - function - location - clinical considerations - Diagram

Differences between cementum and bone: Definition  -  composition – morphology – vascularity – nourishment – secretion- processes direction – remodelling -  function  -  location  -  cementogenesis  -  osteogenesis  -  clinical considerations - Diagrams.

Intermediate cementum: Cemento dentinal junction - Intermediate cementum layer -  origin – composition-   relationship to dentin - alternate name - location – function

Classify cementum and write about cementogenesis and functions of cementum - classification based on presence of cementocytes & type of fibre present - acellular &cellular  -  fibres in cellular  and acellular cementum - Definition of cementogenesis - Hertwig’s epithelial root sheath  - origin of cementoblast—morphology and function - processes involved in cementogenesis - Development of cellular and acellular cementum - Mineralisation - matrix formation - attatchment of fibres—incremental line formation - cementocytes—Diagram - Functions - Repair - Anchorage  -  Adaptation

Acellular cementum - Define cementum  -  classification based on cementocyte presence - based on the fibre content - Acellular Extrinsic Fibre Cementum - Acellular Afibrillar Cementum - Acellular Mixed Fibre Cementum - location - rate of deposition - sharpey’s fibres   -  incremental lines arrangement - function  -  Diagram.

Compare Osteocytes and Cementocytes - Explain about osteocytes  & Cementocytes – origin – secretion – matrix – period of activity – stimulation – resting – reactivation - lacunae  -  canaliculi - immunocytochemistry – Diagram

  



Maxillary sinus

Maxillary sinus: Definition - developmental aspects (note on: developmental anomalies) - structure and variations, microscopic features, functional importance, clinical considerations – diagram

Structure of maxillary sinus: Definition - shape - size - relation to the surrounding structures (four sides) -   ostium - pneumatization/recess development - diagram - -clinical considerations

Histology of maxillary sinus: Definition - light microscopic structure (epithelium, goblets cells and its functions) - diagram - ultramicroscopic structure (nucleus, cytoplasmic organelles, cilia, basal bodies), -  secretions of epithelial -  secretions from the subepithelial glands (serous acini, mucous acini, myoepithelial cells - nervous stimulation of secretions

Antrum of Highmore: Definition of maxillary sinus - definition/what is ostium or antrum of Highmore? - anatomic location in relation to walls of maxillary sinus - relations with the surrounding structures - functional significance/clinical considerations - diagram

Histology of epithelium of maxillary sinus: Definition - olfactory epithelium - cell types - diagram - goblet cells and their functions - electron microscopic view (cilia, basal bodies, mitochondria, GAGs synthesis, golgi apparatus, endoplasmic reticulum) -  functional significance of the histology of maxillary sinus epithelium/clinical considerations

Anatomy of maxillary sinus: Definition - shape - size - relation to the surrounding structures (four sides) - ostium - pneumatization/recess development - diagram - nerve supply - blood supply and clinical considerations




Pulp
Age changes in pulp: Pulp histology - Regressive changes - cell changes - fibrosis - vascular changes—pulp stones—free –attached & embedded - diffuse calcifications—Diagram.
Pulp stones: Definition—location—classification - etiology  -  true & false denticles - pulp stones  - free - attached - embedded  -  clinical considerations   -  diagram
Cell free and cell rich zone of pulp: Histology of pulp - parts  of surrounding specialized odontogenic region -  Other name of cell free zone -  function of this layer - Composition of cell rich zone—fibroblast, undifferentiated mesenchymal cells, fibres & defense cells - period  of formation – Diagram
Defense cells of pulp: Elaborate on histiocytes,  dendritic cells, plasma cells—function and morphology.
Pulp at periphery: Circumscribed by specialized odontogenic region - cells - zones - function and composition—Diagram.
Pulp calcifications:  Pulp stones and diffuse calcifications  -  Definition—location—classification - etiology  -  true & false denticles - pulp stones  - free - attached - embedded  -  clinical considerations   -  diagram.
Histology of pulp: General features of pulp - coronal & radicular pulp  -  layers of pulp - functions of pulp  -  Histology of pulp - parts  of surrounding specialized odontogenic region -  Other name of cell free zone -  function of this layer - Composition of cell rich zone—fibroblast, undifferentiated mesenchymal cells, fibres & defense cells - period  of formation - Diagram
Zones of pulp: Histology of pulp - parts  of surrounding specialized odontogenic region -  Other name of cell free zone -  function of this layer - Composition of cell rich zone—fibroblast, undifferentiated mesenchymal cells, fibers & defense cells - period  of formation - Diagram.
Mast cells: Origin  -  Morphology  -  composition - function   -  H& E staining  - Clinical considerations( role in inflammation )
Describe histology of pulp: General features of pulp - coronal & radicular pulp  -  layers of pulp - functions of pulp  -  Histology of pulp - parts  of surrounding specialized odontogenic region -  Other name of cell free zone -  function of this layer - Composition of cell rich zone - fibroblast, undifferentiated mesenchymal cells, fibers & defense cells - period  of formation - Diagram



Periodontal ligament

Describe the histology of periodontal ligament and briefly mention its functions: A. Introduction – periodontium / periodontal ligament - B. Histology of periodontal ligament - 1. Cells - synthetic cells (osteoblasts, fibroblasts, cementoblasts) - resorptive cells (osteoclasts,fibroblasts,intracellular degradation, cementoclasts) - progenitor cells - epithelial cell rests of Malassez - - defense cells (mast cells, macrophages and lymphocytes) - 2. Extracellular substance  - fibers (Collagen, Sharpey’s fibers, intermediate plexus, elastic fibers, reticular fibers, secondary fibers, indifferent fiber plexus - ground substance - interstitial tissue - 3. Structures present in connective tissue - blood vessels -         - lymphatic drainage – nerves – cementicles - C. Function – Supportive - Sensory – Nutritive  - Homeostatic - Eruptive    - Physical - D. Age changes, unique features of PDL, clinical considerations

What are the functions of periodontal ligament? : Introduction/what is periodontal ligament - functions in detail: Supportive – Sensory – Nutritive – Homeostatic – Eruptive – Physical -- with representative diagrams

Principal fibers of periodontal ligament: Introduction/what is periodontal ligament - Principal fibers (development, location, attachment and functions of each group of fibers):alveolar crest group - horizontal group -  Oblique group - apical group - interradicular group - -diagram

Cells of periodontal ligament: Introduction/what is periodontal ligament -  Principal cells: Synthetic cells (fibroblast, osteoblast, cementoblast) - Resorptive cells (osteoclasts, fibroblasts and cementoclasts) -  Progenitor cells - defense cells (mast cells, macrophages, eosinophils) -  with representative diagrams for each cell type

Passive eruption: What is passive eruption? -  Stages of passive eruption (physiological and  pathological stages of passive eruption of tooth)  -  note on anatomic and clinical crowns of the tooth. - diagram

Sharpey’s fibers: What are Sharpey’s fibers -- attachment/arrangement -  mineralization -  - transalveolar fibers - non-collagenous proteins associated -  diagram

Fibers of periodontal ligament: Introduction/what is periodontal ligament - Fibers of PDL: Collagen fibers (principal fibers, Sharpey’s fibers, intermediate plexus) - elastic-oxytalan fibers - reticular fibers - secondary fibers - indifferent fiber plexus - diagrams



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