Histochem-1

The technique that provides more exact in situ information about chemical composition of cells	Histochemical techniques
The biochemical probe that best characterizes sugar moieties with glycoproteins	Lectins
The examples of histochemistry quantitative analysis	Microphotocell counter; Double beam recording microdensiometry; Integrating microdensiometry
The techniques that use both quantitative and qualitative analysis of tissue substances	Laser spectroscopy; X-ray spectrophotometry; Electron probe microanalysis
Radioautographic techniques	A technique that elucidate the uptake of chemical substance by different metabolic pathways of different tissues and regions of cytoplasm
Proteoglycans are characterized by	Large molecule; Has a protein core; Large number of glycosaminoglycans attached and composed of non-branching disaccharide units
Glycoproteins are characterized	Has a protein macromolecule core; Fewer carbohydrate moieties;Composed of branching disaccharide units
Laminin is secreted	Epithelial cells
During inflammation / early stages of wound healing there is a histochemically demonstrable	Increase in glycoproteins and proteoglycans
Deficiency of ascorbic acid causes	Loss of collagen molecular stability; Loss of stability of triple of helix; Abnormal immature collagen
Histochemical identification of mucins in salivary glands are based on	Glycosaminoglycan content
Formaldehyde when used as a fixative, it’s ideal concentration is	10%
The commonly used fixative for electron microscopy is	Glutaldehyde
The premise of protein based antigens bind to specific antibodies in employed in	Immunohistochemistry
The presence of a bluish blue opalescence in adult teeth of dentinogenesis imperfecta is associated with localization of	Type III collagen
Developing molars exhibited alkaline phosphatase activity in	Stratum intermedium
In electron microscopy the lower penetrating power of electron beam necessitates	Difference in tissue preparation
The most commonly used method of tissue preparation is	Embedding in paraffin
The thickness of sections cut using paraffin embedded tissue is in the range of	4 to 10 micrometers
The thickness of ground sections is in the range of	About 50  micrometers
The most commonly used dental fixative is	10% neutral formalin
Fixation of tissues helps to	Coagulate the protein; Prevents decomposition; Increases permeability
The time required for fixation of a human tissue specimen is a factor(s) of	Size of tissue; Density of specimen; Type of fixative solutions
The aim of dehydration is to	Substitute water with alcohol so paraffin to diffuse into the tissue
While dehydration of tissue during processing, initially the tissue is subjected to	Increasing percentage of alcohol
The processing solution that is miscible with paraffin is	Xylene
The Meyers adhesive is made of	Egg albumin and glycerin
To study pulp it should be ideally fixed with the fixative	Apex opened and immersed in fixative
The ways to determine end point of decalcification are	Piercing with needle; Precipitation test; Radiographic method
The best way to determine end point of decalcification is	Precipitation test
Blocks of parlodion should always be stored in	Alcohol
Parlodion is purified	Nitrocellulose in ether- alcohol
Parlodion embedded sections are cut in	Sliding microtome